TMT-Based Quantitative Proteomics Analysis of the Fish-Borne Spoiler Shewanella putrefaciens Subjected to Cold Stress Using LC-MS/MS

Shewanella putrefaciens is a specific spoilage bacterium for fish during cold storage. To better understand the molecular mechanisms of stress adaptation S. putrefaciens, tandem mass tag- (TMT-) based quantitative proteomic analysis was performed to detect effects on protein expression profiles in which had been cultivated at 4°C and 30°C, respectively. A total 266670 peptide spectrum matching numbers were quantified proteins after data analysis. Of 2292 quantitatively analyzed, 274 found be differentially expressed (DE) under compared with nonstress control. By integrating results Kyoto Encyclopedia Genes Genomes (KEGG) analyses, 9 common KEGG terms notable cold-responsive proteins. Generally, DE involved carbohydrate, amino acid, fatty acid biosynthesis metabolism significantly upregulated, leading energy conservation survival mode. The related DNA repair, transcription, translation implicating change gene more needed response stress.